Category: Hplc
How much HPLC vial size? how much Injection has taken
2 ml vial and 800 micro liter Injection
System suitability parameter in HPLC
% RSD(Related standard deviation)/repeatability
Column efficiency / Theoretical plate
Resolution
Peak to valley ratio
Capacity factor
Signal to noise ratio (S/N ratio)
References standard check (Similarity factor or SF factor)
Retention time
#hplc
What is RRT and RRF in HPLC
Relative retention time
Use:
RRF: Relative responses factor
Use: corrected the differences in detector response of Impurity with analytical peak
RRF Calculation are different AS Per USP and ph.Eup is defined bellow
AS per USP
Sample area x Dilution STD x P x 100
Standard area x Test Dilution x F
F= factor Terminology used
AS per Ph.Eup
Sample area x Dilution STD x P x CF
Standard area x Test Dilution x 100
CF = correction factor terminology
Guide line ICH Q3(A)
Two method About Kown Impurity
External reference standards or relative response factors: some considerations
Different between RT and RRT
RT means Retention time
RRT means Relative Retention time
Relative retention time mean as compared main peak
Example Main peak RT is 5 and A impurities RT is 2
So RRT =2/5 =0.4
Impurities A RRT =0.4
Mobile phase calculation ?
Preparation : Flow rate X Run time X No of injection X +200 ml
= 0.5 X 20 mint X 10 + 200
=300 ml
Diluent : 50 ml 2 standard required
50 X 2 = 100 ml
Total Mobile Phase 400 ml
How many type of chromatography
Liquid chromatography
Gas chromatography
What is usp general chapter?
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What is usp general chapter?
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Hplc columns company
80,000 HPLC columns from more than 30 quality manufacturers and brands
Mostly useable brands given bellow
Agilent Technologies,
Grace,
Merck Millipore,intensil
Thermo Scientific, or Waters. As a high-quality and cost-effective equivalent we offer HLPC columns of our own brand of Altmann Analytik HPLC columns.
Explain part of HPLC
Pump, Sample injector, Coloum, Detector, Recorder, Mobile phase are involve
Explain principle of HPLC
it is a technique of Separation of mixture in to individual compound on base of adsorption Partition , hear stationary phase is ion Exchange and size exclusion principle and mobile phase use
Use of HPLC : Identification , Quantification, Purification ,Testing use of this technique
HPLC training pump
Fluctuating retention times
Unstable Pressure, check pressure section
If mobile phase is not pre-mixed, there could be problem with the ratio been properly mixed(Inconsistent on-line mobile phase mixing)
Unstable Column temperature
Increasing Retention Times
Decreasing Flow rate
Decreasing column temperature
Decreasing Retention Times
Increasing flow rate
Increasing column tempera
Chemistry Interview 